Chameleon: A Software Infrastructure and Testbed for Reliable High-Speed Networked Computing

Coordinated Science Laboratory was formerly known as Control Systems LaboratoryNASA / NAG 1-61

An Experimental Evaluation of the REE SIFT Environment for Spaceborne Applications

Coordinated Science Laboratory was formerly known as Control Systems Laborator

Analysis of Credential Stealing Attacks in an Open Networked Environment

Abstract. This paper analyses the forensic data on credential stealing incidents over a period of 5 years across 5000 machines monitored at the National Center for Supercomputing Applications at the University of Illinois. The analysis conducted is the first attempt in an open operational environment (i) to evaluate the intricacies of carrying out SSH-based credential stealing attacks, (ii) to highlight and quantify key characteristics of such attacks, and (iii) to provide the system level characterization of such incidents in terms of distribution of alerts and incident consequences. Keywords-Incident analysis;Credential stealing; Intrusion detectio

Improved selectivity of an engineered multi-product terpene synthase

Mutation of the sesquiterpene synthase Cop2 was conducted with a high-throughput screen for the cyclization activity using a non-natural substrate. A mutant of Cop2 was identified that contained three amino acid substitutions. This mutant, 17H2, converted the natural substrate FPP into germacrene D-4-ol with 77% selectivity. This selectivity is in contrast to that of the parent enzyme in which germacrene D-4-ol is produced as 29% and α-cadinol is produced as 46% of the product mixture. The mutations were shown to each contribute to this selectivity, and a homology model suggested that the mutations lie near to the active site though would be unlikely to be targeted for mutation by rational methods. Kinetic comparisons show that 17H2 maintains a k_(cat)/K_M of 0.62 mM^(−1) s^(−1), which is nearly identical to that of the parent Cop2, which had a k_(cat)/K_M of 0.58 mM^(−1) s^(−1)

Incorporating Reconfigurability, Error Detection and Recovery into the Chameleon ARMOR Architecture

Coordinated Science Laboratory was formerly known as Control Systems LaboratoryJet Propulsion Lab / NASA JPL 96134

High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase

The development of high-throughput assays can be extremely challenging, yet is essential for many applications in drug discovery and enzyme engineering